Dear Georg,
There are different ways to set-up counterdiffusion experiment but the
set-up you mentioned is done in a single capillary. The easiest way is to
prepare the agarose and when it is still warm pipette the 100 microL on any
surface, I use a piece of parafilm. Them you have to adsorb it wi
Hello Georg,
You can melt the agarose in a glass container using a microwave oven. Then
connect your capillary tube to small syringe, using a plastic tubing or
adapter. Use some parafilm if the tubing diameters do not fit exactly,
there should be no leaks. Then slowly pull the plunger to take ~100
Hi,
Warmed up capillary and hamilton syringe works fine (i used heat lamps
similar to pizza heaters used by all-night pizza joints). You can get a
cheap heat lamp from most pet stores (terrarium heaters).
Artem
On Jan 5, 2015 11:32 AM, "Georg Mlynek" wrote:
> Dear Colleagues,
>
> After reading
https://hamptonresearch.com/product_detail.aspx?cid=10&sid=65&pid=109
Granada box will work for you
Thaumatin can be grown to large single crystals suitable size
On Mon, Jan 5, 2015 at 9:25 AM, Georg Mlynek
wrote:
> Dear Colleagues,
>
> After reading a few papers about growing suitable crystal
Hi Georg,
I haven't used the agarose method, but have sealed many capillaries using bees
wax without an issue.
Simply heat the wax until it melts, dip the capillary (rotate if it has a
larger diameter) then let cool.
I hope that helps, cheers!
Sean Seaver, PhD
P212121
http://store.p212121.co
