to:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Roger
Rowlett
Sent: Thursday, July 19, 2012 4:42 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Protein concentration vs Molecular wt...
This is almost exactly our basic approach, too. Before we got a dropsetter, we
did 24 wells (1/2 screen) to get a fee
See http://www.douglas.co.uk/PDB_data.htm, section 4
On 19 July 2012 20:58, james09 pruza wrote:
> Dear Crystallographers,
> Is there any rule of thumb for Protein concentration and molecular weight
> for crystallization trials of a soluble protein? Looking for high molecular
> wt. protein ~50
Hi James,
You can get the PCT (Pre-Crystallisation Test) from one of the more
famous manufacturers of crystallography products - essentially you can
quickly screen your protein to get an idea if it is too dilute, too
concentrated, or somewhere in the middle. Of course, the results are
representati
This is almost exactly our basic approach, too. Before we got a
dropsetter, we did 24 wells (1/2 screen) to get a feel for the correct
protein concentration. Some additional rules of thumb we use:
1. We usually start at 10 mg/mL protein and go up or down from there
depending on the results o
I don't think there is such a rule, but in the old days, when we only had
Hampton Screen I and II, the rule was:
- Set up screen 1, look at the drops and you should expect some kind of
precipitation in 50% of the drops. If much less than that, increase your
protein concentration. If much more t