Hi James, You can get the PCT (Pre-Crystallisation Test) from one of the more famous manufacturers of crystallography products - essentially you can quickly screen your protein to get an idea if it is too dilute, too concentrated, or somewhere in the middle. Of course, the results are representative only (I've had samples which looked great on the PCT but then gave me ~90% clear drops even at 40 mg/ml, and an 80 kDa protein which crystallised at 2 mg/ml) so as with anything they should be taken with the appropriate vessel full of sodium chloride...
If you have enough protein, then I agree with the previous comments that it is more useful to set up some trays and see what % of conditions give hits, and work from there. Cheers, Tom On 19 July 2012 20:58, james09 pruza <james09x...@gmail.com> wrote: > Dear Crystallographers, > Is there any rule of thumb for Protein concentration and molecular weight > for crystallization trials of a soluble protein? Looking for high molecular > wt. protein ~50kDa. > James. > -- Skype: tom.murray.rust Twitter: tmurrayrust http://twitpic.com/photos/tmurrayrust +44 7970 480 601 (UK)
