To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Bubbles on crystals
Perhaps the bubbles are related to the bubbles which form when mixing
alcohols with water, e.g., when making coomassie destain? And what are those
bubbles, by the way?
Jacob
***
Jacob Pear
Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
***
- Original Message -
From:
To:
Sent: Monday, July 06, 2009 5:56 PM
Subject: Re: [ccp4bb] Bubbles
Hi,
If you're worried - instead of transfering the crystal, just flood the
entire drop with excess of your cryo buffer. No exposure to air this way.
I bet that the bubbles won't go away though :)
Artem
> Dear members,
> I have protein crystals grown in a condition with Isopropanol (13%) and
> ci
Have you tried to grow the crystals in ethylene glycol or glycerol instead
of isopropanol?
On Mon, 6 Jul 2009, kumar wrote:
Dear members,
I have protein crystals grown in a condition with Isopropanol (13%) and
citric acid (0.2 M). When I transfer the crystals to cryo-buffer (30%
glycerol or 2
