Hi, If you're worried - instead of transfering the crystal, just flood the entire drop with excess of your cryo buffer. No exposure to air this way. I bet that the bubbles won't go away though :)
Artem > Dear members, > I have protein crystals grown in a condition with Isopropanol (13%) and > citric acid (0.2 M). When I transfer the crystals to cryo-buffer (30% > glycerol or 25% ethyleneglycol with 0.25 M citric acid and 13%-15% > Isopropanol), small bubbles appear on the surface of the crystals. These > tiny bubbles sometimes disappear in few seconds and sometimes not. > Crystals > are exposed to air only for 2-3 seconds in the process of transfer. I am > worried if the formation of these bubbles is due to evaporation of > isopropanol form the crystal and if it affects the crystal lattice. These > crystals diffract X-rays upto 4 angstroms. Any ideas how to avoid this > problem? > Thank you > Kumar >
