Hi,
similarly, 30 sec soak with 0.5 M potassium iodide. But in this case,
the iodides bound specifically to a site where otherwise chloride
binds. These halide binding sites were totally unexpected ...
Best regards,
Dirk.
Am 26.09.2007 um 01:55 schrieb James Whisstock:
Hi - sorry - rath
Dear Derek and Tassos,
I normally start using the following compounds depending on pH of the
cryo-solution at 10 mM concentration and 10 min incubation time and
optimize towards lower concentrations and longer incubation times:
-ethyl mercuric phosphate (EMP)
-HgCl2
-SmCl3
-K2PtCl4
-K2PtCl6
Be
I also solved a structure on our home CuKa source by SIRAS with 20-60sec in
0.5-1M KI.
JPK
==Original message text===
On Tue, 25 Sep 2007 6:55:28 pm CDT James Whisstock wrote:
Hi - sorry - rather than iodine I meant to say we had had success with
Potassium Iodide (1M f
Hi - sorry - rather than iodine I meant to say we had had success with
Potassium Iodide (1M for 20 seconds)!
Cheers
James
[EMAIL PROTECTED] wrote:>
> Hi,
>
> I do not use their method as such - however, I love heavy atom soaks and
> do them any time I can, so I've got very similar experience
Hi,
I do not use their method as such - however, I love heavy atom soaks and
do them any time I can, so I've got very similar experiences in the past.
Heavy atoms can bind very quickly even from quite dilute solutions - the
quickest I've ever soaked (and got useful data) was sodium chloroplatinat
Hi Derek
We have had success with iodine - 20 seconds soak.
J
Derek Logan <[EMAIL PROTECTED]> wrote:>
> Hi Uwe,
>
> Just what I wanted to hear, and with a limited set of compounds too!
> Follow-up question: what are these 4-6 most successful compounds?
> Thanks also for the tips on monitoring
There's a nice databank out there in the world wild web:
http://www.sbg.bio.ic.ac.uk/had/
Juergen
Derek Logan wrote:
Hi Uwe,
Just what I wanted to hear, and with a limited set of compounds too!
Follow-up question: what are these 4-6 most successful compounds?
Thanks also for the tips on
Hi,
I've had a couple of successful cases:
1) Uranyl acetate soak to get starting phases at 6.0A combined with a gold
derivative at 3.8A. Both quick soaks of about 10-20 mins in 5-10 mM on crystals
less than 100 microns.
2) Mercuric chloride soak of 10 mM for 10 mins to get starting phases
Hi Uwe,
Just what I wanted to hear, and with a limited set of compounds too!
Follow-up question: what are these 4-6 most successful compounds?
Thanks also for the tips on monitoring thw soaking
Derek
On Sep 25, 2007, at 13:23, Uwe Mueller wrote:
Dear Derek,
we are using the quick-soak me
You may also wish to take a look in Dauter and Nagem's quick cryo
soaking for introducing anomalous scattering.
1. Acta Crystallogr D Biol Crystallogr. 2001 Jul;57(Pt 7):996-1002.
2. Methods Enzymol. 2003;374:120-37.
3. Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1022-30
HTH
[ ]s
-
Dear Derek,
we are using the quick-soak method frequently at the BESSY-MX beamlines.
So far we were able to obtain phase information in terms of MAD/SAD-
phases in all cases which we tried. The starting set-up is the screen
over the 4-6 most successful heavy atom compounds at 5-10mM
concentrat
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