Re: [ccp4bb] water soluble protein that needs detergent to be stable

I have not dealt myself with this type of protein, but Alex McPherson tested the detergent beta-octylglucoside (at a concentration of 1.5 %, i.e. above the cmc) for the crystallisation of soluble protein and tRNA (1986, J.Biol.Chem 261:1969-75), the detergent did not hampered the crystallisation, a

Re: [ccp4bb] water soluble protein that needs detergent to be stable

otein to be more soluble, etc. etc. Artem _ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Daniel Jin Sent: Tuesday, August 28, 2007 1:45 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] water soluble protein that needs detergent to be stable Hi, I am working on a 60 k

[ccp4bb] water soluble protein that needs detergent to be stable

Hi, I am working on a 60 kDa C. elegans protein that is predicted to be mostly alpha-helix. It is over-expressed in E.coli and the yield is about 1 mg/L of cell culture. The CD spec at 4 degree showed the presence of dominant alpha-helix. However, we don’t have any functional assay to conf