Dear Rui,
I can only support Patrick's view on MMS. We've got some very good
results with this technique in one tricky case in which the starting
point wasn't much better than yours.
In addition to the papers Patrick suggested the following might also
be worth a look:
The role of bias in
k] On Behalf Of
rui
Sent: 25 August 2010 13:37
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] turn granular to crystal
Hi, All,
I'm trying to crystallize a soluble protein and got something like
granular, they are rounded shaped and not so regular and also don't have
sharp edges. See the a
--
> yybbll
>
> *发件人：* rui
> *发送时间：* 2010-08-27 02:04:54
> *收件人：* yybbll
> *抄送：*
> *主题：* Re: [ccp4bb] turn granular to crystal
> hi,
>
> no , I didn't use detergent. THe composition of the well buffer is
> just
rui
发送时间： 2010-08-27 02:04:54
收件人： yybbll
抄送：
主题： Re: [ccp4bb] turn granular to crystal
hi,
no , I didn't use detergent. THe composition of the well buffer is just PEG4000
and MgCl2 and NaAc.
On Wed, Aug 25, 2010 at 9:52 AM, yybbll wrote:
Hi, Did you use detergent? It
I would try the following approach, some of
which you may have done already:
Broad pH screen, by 0.5 pH units to see if you are in the pH
sweet spot.
Finer PEG-4000 screen at the pH identified in the pH
screen--the precipitate in the we
Hi Rui,
I would try the additive screen.
http://hamptonresearch.com/product_detail.aspx?cid=1&sid=36&pid=27
Best Regards,
Georg
rui schrieb:
Hi, All,
I'm trying to crystallize a soluble protein and got something like
granular, they are rounded shaped and not so regular and also don't
have
Hi, Did you use detergent? It seems look like detergent crystals.
2010-08-25
yybbll
发件人： rui
发送时间： 2010-08-25 20:37:18
收件人： CCP4BB
抄送：
主题： [ccp4bb] turn granular to crystal
Hi, All,
I'm trying to crystallize a soluble protein and got something like granular,
they are ro
