Hi, You can easily test the crystals by adding a drop of "Ponceau S" staining solution. If you have protein crystals they will get stained. Best wishes, Tim
yybbll wrote: > Hi, > A little danger this condition. I suggest you should firstly make sure > they are protein crystals. I know it is very difficult. I suggest you > can add proteases to the drops. If "crystals" dissolve, no problem, > they are protein crystals. > Best > Yibin > 2010-08-27 > ------------------------------------------------------------------------ > yybbll > ------------------------------------------------------------------------ > *发件人：* rui > *发送时间：* 2010-08-27 02:04:54 > *收件人：* yybbll > *抄送：* > *主题：* Re: [ccp4bb] turn granular to crystal > hi, > > no , I didn't use detergent. THe composition of the well buffer is > just PEG4000 and MgCl2 and NaAc. > > On Wed, Aug 25, 2010 at 9:52 AM, yybbll <yyb...@gmail.com > <mailto:yyb...@gmail.com>> wrote: > > Hi, Did you use detergent? It seems look like detergent crystals. > 2010-08-25 > ------------------------------------------------------------------------ > yybbll > ------------------------------------------------------------------------ > *发件人：* rui > *发送时间：* 2010-08-25 20:37:18 > *收件人：* CCP4BB > *抄送：* > *主题：* [ccp4bb] turn granular to crystal > Hi, All, > > I'm trying to crystallize a soluble protein and got something like > granular, they are rounded shaped and not so regular and also > don't have sharp edges. See the attached pic. The current > condition is PEG4000 and pH around 5. How can I improve this > condition? Thanks a lot. > > > -- Tim Schulte Ruhr-Universita"t Bochum LS Biophysik AG Proteinkristallographie Universita"tsstr. 150 44780 Bochum Germany Tel. +49-234-32-25754
