Hi,
one could indeed get much better phases with the correct positions of
the HA cluster. If there is sufficient anomalous signal one could use
the cluster as search model for Molecular replacement using the
anomalous data as described in Dahms et al. to get the exact position
of the cluste
Dear Lisa,
You are not saying how far your data extends in resolution.
In my experience, finding the cluster(s) can work better if you limit your data
used in the
search ~5A, as scattering goes down beyond this due to interference between
atoms of the clusters.
It does come back at higher scat
If you set the atom type to "TX", phaser will use the built-in scattering
factors for the tantalum bromide cluster. This will be represented as a point
scatterer rather than the actual cluster - if you have very good data you may
be able to actually position the tantalum atoms in the cluster an
Dear Lisa,
[there is a SHARP discussion list at
http://www.globalphasing.com/mailman/listinfo/sharp-discuss]
On Thu, Nov 21, 2013 at 02:29:23PM +0800, LISA wrote:
> Dear All,
>
> I am running autosharp with a single wavelength data soked with Ta6Br12.
> This data collected at the wavelength of 1
Dear Lisa,
You could try using SHELXD (e.g. via hkl2map) to find the heavy atoms,
it doesn't need f' and f". However you should take into account that
many soaks have not absorbed the intended heavy atoms or clusters, and
that if a tantalum bromide cluster has actually been incorporated it
will pr
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Dear Lisa,
the wavelength indicates you collected the data at a synchrotron. Do
you have a fluorescence scan of your crystal? That should tell you f'
and f''.
Other than that you can use Ethan Merritt's excellent server at
http://skuld.bmsc.washingto
Dear Dr. Matthias Zebisch,
how to do the specific cluster search in phaser sad pipline? Thank you.
Lisa
On Thu, Nov 21, 2013 at 3:37 PM, Matthias Zebisch <
matthias.zebi...@bbz.uni-leipzig.de> wrote:
> Dear Lisa,
>
> if you have proper anomalous data I rather recommend using the Phaser SAD
> p
Dear Lisa,
if you have proper anomalous data I rather recommend using the Phaser
SAD pipeline and specify cluster search.
Worked instantly for me.
Good luck!
-
Dr. Matthias Zebisch
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics
Dear All,
I am running autosharp with a single wavelength data soked with Ta6Br12.
This data collected at the wavelength of 1.254A. I told the autosharp the
f' -20 and f''10.5. The autosharp result said these values are not correct?
How can we get the f' and f'' of this cluster? Thank you.
Lisa
