If you set the atom type to "TX", phaser will use the built-in scattering factors for the tantalum bromide cluster. This will be represented as a point scatterer rather than the actual cluster - if you have very good data you may be able to actually position the tantalum atoms in the cluster and get better phase information that way.
Jason. -- Jason Busby PhD Student Laboratory of Structural Biology School of Biological Sciences University of Auckland Thomas Building 110 3a Symonds St Private Bag 92019 Auckland 1142 New Zealand ph: +64 9 3737599 ext 84155 fx: +64 9 3737414 On 21/11/2013, at 9:32 PM, LISA wrote: Dear Dr. Matthias Zebisch, how to do the specific cluster search in phaser sad pipline? Thank you. Lisa On Thu, Nov 21, 2013 at 3:37 PM, Matthias Zebisch <matthias.zebi...@bbz.uni-leipzig.de<mailto:matthias.zebi...@bbz.uni-leipzig.de>> wrote: Dear Lisa, if you have proper anomalous data I rather recommend using the Phaser SAD pipeline and specify cluster search. Worked instantly for me. Good luck! ----------------------------------------- Dr. Matthias Zebisch Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK Phone (+44) 1865 287549; Fax (+44) 1865 287547 Email matth...@strubi.ox.ac.uk<mailto:matth...@strubi.ox.ac.uk> Website http://www.strubi.ox.ac.uk<http://www.strubi.ox.ac.uk/> ----------------------------------------- On 11/21/2013 6:29 AM, LISA wrote: Dear All, I am running autosharp with a single wavelength data soked with Ta6Br12. This data collected at the wavelength of 1.254A. I told the autosharp the f' -20 and f''10.5. The autosharp result said these values are not correct? How can we get the f' and f'' of this cluster? Thank you. Lisa
