[ccp4bb] Test message

This is a test message to explore issues reported about automatic removals from the BB. -- David To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=

[ccp4bb] Test the new sequence and ligand components at PDBe

Dear CCP4BB We are delighted to offer two new components available for beta testing on our PDBe entry pages: the ProtVista sequence component on the PDBe macromolecules pages and the LigEnv component on the ligands and environments pages. The ProtVista sequence view component has replaced o

Re: [ccp4bb] test

Sent: Tuesday, November 26, 2019 11:47 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] test just running a test, haven't been receiving my own messages... Jeffrey B. Bonanno, Ph.D. Department of Biochemistry Albert Einstein College of Medicine 1300 Morris Park Avenue Bronx, NY 10461 off. 71

[ccp4bb] test

just running a test, haven't been receiving my own messages... Jeffrey B. Bonanno, Ph.D. Department of Biochemistry Albert Einstein College of Medicine 1300 Morris Park Avenue Bronx, NY 10461 off. 718-430-2452 fax. 718-430-8565 email jeffrey.bona...@einstein.yu.edu

[ccp4bb] Test system for binding energy predictions

Dear crystallographers - I've been trying without success to find a suitable system to test a method for predicting change in binding energy (delta-delta-G) due to mutations of protein-protein complexes that result in a change in net charge. In particular I'm looking for a system with the fol

[ccp4bb] Test

This is a test Sent from my Windows Phone

[ccp4bb] test

Dear BB, sorry for the email I’m just validating that after 6 months abstinence I’m back on the CCP4BB and can actually post. Jürgen .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malari

[ccp4bb] Test [My apologies]

My apologies to all for this apparent spam, but one of my colleagues signed up for the CCP4BB recently and despite Ronan Keegan checking that his account has been activated, he has not been receiving CCP4B email. I'm trying to help him track down the problem with this email. __

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

You can put your "dogbowl dewar" under the microscope and focus it on the surface of the liquid nitrogen. This works very well once you have got the lighting right. You want to shine the light on the bottom of the dewar so that it acts as a backlight for your loop. You can hold the loop just

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

Checking Cryo-Conditions under the microscope can actually be very useful. Even if you have access to a X-ray beam, simple pre-tests can be very informative. This can be done as follows: A 24well plate cover is placed on a stereo microscope open side down, a second one on top of it the oth

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

I think I posted this link maybe two years ago: http://idb.exst.jaxa.jp/db_data/protein/search-e.php? Jürgen On Sep 22, 2009, at 12:48 PM, Elspeth Garman wrote: or see concentrations given in: McFerrin and Snell J.Appl.Cryst (2002) 35, 538 and Mitchell and Garman, J.Appl.Cryst. (1996) 29, 584

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

or see concentrations given in: McFerrin and Snell J.Appl.Cryst (2002) 35, 538 and Mitchell and Garman, J.Appl.Cryst. (1996) 29, 584 Good luck EFG Paul Leonard wrote: Hi Claudia It really is best to test the cryo condition in an X-ray beam. However, if you don't have access to X-rays in hous

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

Hi Claudia It really is best to test the cryo condition in an X-ray beam. However, if you don't have access to X-rays in house you could look at the hampton crystallization screen conditions (or another supplier). Find a condition with a similar amount and type of precipitant as you have in your

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

It might be risky (and silly), but I have done this successfully. I just put a small dewar of liquid N2 under the scope, focussed directly above the surface and looked to see if cryo solutions froze clear or not. The ones that did worked out when we got to the beamline (we'd verified that

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

Hi Claudia "By eye" is not a bad approximation. The trick I learnt at the JCSG (I forget the originator, sorry) is to suck up some solution in a P2 pipette tip (the clear ones), dip it in lN2, and then hold it just above the lN2 (where it's still cold) to check for clearness or not. Is a go

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

Dear Claudia, please be so kind and tell us all what is / are the crystallization condition(s)? It would help to us to answer your question! - J. - Claudia Scotti wrote: Dear List, Sorry for the probably silly question. Any suggestions to test cryoconditions without X-rays or cryostre

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

I have not heard of anyone checking for suitable cryo in that fashion how fast can you do it before it is affected by room temp and how would you know about the ice rings, but when you have crystal(s) and not crystal you can freeze different ones in several different cryos (sugars, glycerol etc) an

[ccp4bb] Test of cryoconditions without X-rays or cryostream

Dear List, Sorry for the probably silly question. Any suggestions to test cryoconditions without X-rays or cryostream? I'd need to freeze crystals before going to ESRF and I'm a bit anxious. Is it enough to try to freeze the cryoconditions in liquid nitrogen checking under the mi

Re: [ccp4bb] test data sets part II...

Hi Mark We are more than happy to help you setup a local repository - its fairly painless and cheap. Otherwise - we'll host your data - we have the solution worked out pretty much here and have moved on quite a bit from the Androulakis paper - see http://www.tardis.edu.au. It might cost yo

Re: [ccp4bb] test data sets part II...

Dear All, with regards to diffraction images storage (see also Androulakis et al., Acta Cryst. (2008). D64, 810–814: "Federated repositories of X- ray diffraction images"), I am all for it - one to be able to catch fraud, but more importantly so structures can be improved in the future and

Re: [ccp4bb] test data sets part II...

EMBL-Hamburg and BESSY have put a number of interesting data-sets including tutorial materials for data processing and phase determination made available recently. You can download the data from both locations: http://www.mx.bessy.de/xray_tutorial.shtml http://webapps.embl-hamburg.de/Xray_Tutor

Re: [ccp4bb] test data sets part II...

I have put some data sets, and data reductions including crystallographic calculations, into XDSwiki: check out http://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Quality_Control This is work in progress, so expect additions/changes! And the best is: anyone may contribute ... HTH, Kay

Re: [ccp4bb] test data sets part II...

We put our raw data, plus metadata here: http://www.tardis.edu.au JCSG also make data available, but I think its just an archive, with little or no annotation cheers Ashley On 24/09/2008, at 7:02 AM, afyfe wrote: Dear Tommi, I'm not sure whether 'test data sets part I' relates to the query

Re: [ccp4bb] test data sets part II...

Dear Tommi, I'm not sure whether 'test data sets part I' relates to the query I mailed the other day but, in light of the recent posts regarding fundamental literature, your suggestion seems an excellent one. Perhaps I'm not looking in the right places, but finding examples of image sets

[ccp4bb] test data sets part II...

Also, i think that would be nice if this type of info could be put on the web, part of the wiki for instance.. if there is some consensus to what works + the typical proteins easily available. Tommi Kajander, Ph.D. Structural Biology and Biophysics Institute of Biotechnology University of Hel

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[ccp4bb] Test. Please ignore