Hi Claudia It really is best to test the cryo condition in an X-ray beam.
However, if you don't have access to X-rays in house you could look at the hampton crystallization screen conditions (or another supplier). Find a condition with a similar amount and type of precipitant as you have in your crystallisation condition and look up how much glycerol they added as cryoprotectant. Then try making up your own crystallisation condition with the amount of glycerol they recommend and freeze your crystals in that solution. Also try crystal soaks of varying lengths of time (30 seconds - 5 min) in the cryo condition as well as trying to soak some crystals where you gradually increase the %glycerol in the crystal soak prior to freezing (in 2% glycerol steps for example). see http://hamptonresearch.com/product_detail.aspx?cid=1&sid=20&pid=3 Good luck. Paul > > > > Dear List, > > > > Sorry for the probably silly question. > > > > Any suggestions to test cryoconditions without X-rays or cryostream? > > > > I'd need to freeze crystals before going to ESRF and I'm a bit anxious. Is > it enough to try to freeze the cryoconditions in liquid nitrogen checking > under the microscope (or by eye) or is this still risky? > > > > Thanks, > > > > Claudia > > > > > > Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia > Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. > 0039 0382 986335/8/1 Facs 0039 0382 303673 > > > > _________________________________________________________________ > With Windows Live, you can organize, edit, and share your photos. > http://www.microsoft.com/middleeast/windows/windowslive/products/photo-gallery-edit.aspx Department of Biochemistry UMDNJ-Robert Wood Johnson Medical School Center for Advanced Biotechnology and Medicine 679 Hoes Lane Piscataway, New Jersey 08854-5627 Phone: (732) 235-4206 Email: leon...@cabm.rutgers.edu
