Crush, pippet up and down, dilute as needed (I used 1:200) and vortex.
Actually anything works... as everything - protein dependent.
For the stabilizing solution I have used the same as the reservoir
solution (20%MPD, 250 mM NaCl, 0.1 M HEPES pH 7.5) or 2% more MPD. Worked
fine and the seeds were
Hi,
in this paper
http://journals.iucr.org/d/issues/1999/05/00/gr0904/gr0904.pdf
they seemed to use the protein solution as a stabilising solution. Seems
like a good idea as you are not diluting your concentrations too much in
that case.
cheers
charlie
> Dear CCP4bbers,
>
> Please comment on
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From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
james09 pruza
Sent: 06 September 2009 09:14
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] stabilizing solution
Dear CCP4bbers,
Please comment on the stabilizing solution for seed s
Dear CCP4bbers,
Please comment on the stabilizing solution for seed stocks. If the crystal
is in 30% MPD and coming after 2-3 days, what should be the stabilizing
solution for seeding.
Thanks.
James
