James
Generally you can use the reservoir solution to make your seed stocks, as is routinely done with the "MMS" microseeding method. However, the seeds will not be completely stable because there will not be much protein in the solution, so you must keep your seed stocks on ice, and freeze them as soon as you've finished using them I imagine that MPD would stabilize your seed crystals as well as anything else Reference below Best wishes Patrick [2] Allan D'Arcy, Frederic Villarda, May Marsh. 'An automated microseed matrix-screening method for protein crystallization'. Acta Crystallographica section D63 (2007), 550-554. On-line at http://scripts.iucr.org/cgi-bin/paper?S0907444907007652 -- For information and discussion about protein crystallization and automation, please join our bulletin board at http://groups-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.uk Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of james09 pruza Sent: 06 September 2009 09:14 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] stabilizing solution Dear CCP4bbers, Please comment on the stabilizing solution for seed stocks. If the crystal is in 30% MPD and coming after 2-3 days, what should be the stabilizing solution for seeding. Thanks. James
