ase
> *Sent:* Tuesday, June 09, 2009 1:56 PM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] soaking problems
>
> Dear all,
>
> I tried to soak substrate (sugar) into my enzyme-crystals. After solving
> the structure I found PEG molecules instead of substrate in the active
>
Sent: Tuesday, June 09, 2009 1:56 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] soaking problems
Dear all,
I tried to soak substrate (sugar) into my enzyme-crystals. After
solving the structure I found PEG molecules instead of
Dear all,
I tried to soak substrate (sugar) into my enzyme-crystals. After solving the
structure I found PEG molecules instead of substrate in the active site. Does
this mean that the active site is blocked and the substrate isnĀ“t able to get
into the enzyme-crsytals anymore? I used PEG 1000 i