Re: [ccp4bb] soaking problems

2009-06-09 Thread Matthew Chu
ase > *Sent:* Tuesday, June 09, 2009 1:56 PM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] soaking problems > > Dear all, > > I tried to soak substrate (sugar) into my enzyme-crystals. After solving > the structure I found PEG molecules instead of substrate in the active >

Re: [ccp4bb] soaking problems

2009-06-09 Thread Herman . Schreuder
Sent: Tuesday, June 09, 2009 1:56 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] soaking problems Dear all, I tried to soak substrate (sugar) into my enzyme-crystals. After solving the structure I found PEG molecules instead of

[ccp4bb] soaking problems

2009-06-09 Thread Marek Frischerkase
Dear all, I tried to soak substrate (sugar) into my enzyme-crystals. After solving the structure I found PEG molecules instead of substrate in the active site. Does this mean that the active site is blocked and the substrate isnĀ“t able to get into the enzyme-crsytals anymore? I used PEG 1000 i