Indeed nothing is predictable. We can only assume things on what is *planed*
and *promised*, and you can take my words on the fact that I will do my best to
have it done properly and as close to the expectations as possible. But again,
nobody can really predict the future.
Finger crossed.
Cheer
'Will' is a fashionable word - but since even a Nobel prize was given
based on promises never kept, who would be surprised...
Best wishes from now,
Tim
On 12/10/2013 10:11 AM, CHAVAS Leonard wrote:
> Yes, Tim is right… for now… in few years, with the E-XFEL, we'll get to much
> less sample, and
Yes, Tim is right… for now… in few years, with the E-XFEL, we'll get to much
less sample, and much more time available. But that's in few years.
Leo
On Dec 10, 2013, at 10:01 AM, Tim Gruene wrote:
> Dear Careina,
>
> you can also apply for beamtime at PETRA-III. You get away with the same
> s
Dear Careina,
you can also apply for beamtime at PETRA-III. You get away with the same
size crystals but only require a nano liter drop rather than a few ml of
your sample. And you probably get beamtime much quicker because all the
equipment is installed and collecting a data set takes very short
Dear Jens, Careina
this is not really an *advantage*, but rather a *convenience*. You can still
use big crystals if you'd like to, but as they usually never survive more than
one shot (few femtosec), you'd need a lot of these bigger crystals to collect a
full data.
And yes, I would also highly
Careina,
If your target is interesting enough, try to reproduce the small
crystals in batch and apply for FELS time. Small crystals are actually
an advantage there.
Cheers,
Jens
On Wed, 2013-12-04 at 21:41 -0800, Careina Edgooms wrote:
> Hi all
>
>
> Any advice on how to get bigger crystals
Hi,
sorry, it should read "salt in" not "inverse salt in" ...
- J. -
Am 05.12.13 19:55, schrieb mesters:
Hi,
Showers of crystals often occur if the protein is not that
soluble/happy/stable in the solute. The solubility of a protein
depends on its concentration, its pI, pH of solute, temper
etin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Mahesh Lingaraju
[mxl1...@psu.edu]
Sent: Thursday, December 05, 2013 5:38 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] small crystals
Hi All
On similar lines, I have been trying to optimize crystallization conditions for
my protein. Initia
gt; At what temperature and what precipitant are you using?
>>
>> - Jeroen -
>>
>>
>> showers of crystals
>>
>>
>> Original message
>>
>> Subject:[SURESPAM] [ccp4bb] small crystals
>> From:Careina Edgooms
>> To:CCP4BB@JISCMAIL.
howers of crystals
>
>
> ---- Original message
>
> Subject:[SURESPAM] [ccp4bb] small crystals
> From:Careina Edgooms
> To:CCP4BB@JISCMAIL.AC.UK
> Cc:
>
>
> Hi all
>
> Any advice on how to get bigger crystals from conditions that give
> shower
At what temperature and what precipitant are you using?
- Jeroen -
showers of crystals
Original message
Subject:[SURESPAM] [ccp4bb] small crystals
From:Careina Edgooms <car
quality. You
can find information on the approach here:
http://pubs.acs.org/doi/abs/10.1021/cg2001442?journalCode=cgdefu
Best regards, Morten
Original message
Subject:[SURESPAM] [ccp4bb] small crystals
From:Careina Edgooms mailto:careinaedgo...@yahoo.com>>
To:CCP4BB@JISCMAIL
Hi all
Any advice on how to get bigger crystals from conditions that give showers of
tiny crystals? I am getting small pretty looking individual crystals but they
are too small and they don't seem to grow. In fact, in some instances if left
for a couple of days they actually dissolve. I have fi
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