Hello Jerry,
We use the Van Duyne et al feedback inhibition method with BL21
and have never had a problem.
ho
UC Berkeley
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Date:Thu, 26 Mar 2009 14:58:57 -0700
From:Jerry McCully
Subject: selenomethionine labeling
--_6abd9eb3-ba7b-436a-99d3-053f7f3a6
lf Of Jerry
McCully
Sent: Thursday, March 26, 2009 5:59 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] selenomethionine labeling
Dear All:
I got a problem with the expression of one N-terminal His6 tagged
protein in B834 cells when I tried to do selenomethionine labeling.
However
Dear All:
I got a problem with the expression of one N-terminal His6 tagged protein
in B834 cells when I tried to do selenomethionine labeling.
However, the protein can be expressed in BL21{DE3) cells.
Welcome any troubleshooting tips.
All the best,
Jerry
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