Thanks to all again! Find below my answers/comments to all your replies.
Colin Nave,
I'll certainly collect higher resolution dataset to look for more
diagnostic rings.
Apo-ferritin xtallizes in the same conditions with the same cell (I know it
from literature), I'll measure it too, to look for di
Dear Anna,
Very interesting diffraction pattern.
Any chance of measuring to higher resolution?
Ie to try and capture the higher order rings, which presumably are there.
Also interesting that these rings seem quite weak ie the ferritin perhaps not
fully loaded?
Best wishes,
John
Prof John R Helli
nes I was fretting about. All
diffraction features (rings or spots) should be indexed otherwise the job is
incomplete!
Colin
From: Jacob Keller [mailto:j-kell...@fsm.northwestern.edu]
Sent: 08 May 2012 18:29
To: Nave, Colin (DLSLtd,RAL,DIA)
Cc: ccp4bb
Subject: Re: [ccp4bb] saxs on xtals
Dear
Seems to me that if you really do have a little magnet inside your
ferritin you would expect the protein crystal to behave like a "spin
glass". Spin glasses are a classic homework problem in statistical
thermodynamics that makes most students question their choice of major.
However, invoking
ngs but you
> should check. Also be nice to know if apoferritin crystallised under the
> same conditions (if it can be) shows these rings
>
> Regards
> Colin
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> anna anna
> Sent: 08 May 2012 16:55
> To:
2 16:55
To: ccp4bb
Subject: Re: [ccp4bb] saxs on xtals
Dear all,
first of all I want to thank you for your attention and all your brilliant
suggestions that really cleared my head!!!
Thanks to you (or because of you!!) now I have many ideas and very much to do.
Colin,
I was just re-considering my d
Dear Anna,
I know that you already have gotten replies from some top experts, but your
intriguing problem brought up some issues I have run across in the past.
First, from you experience with single crystal diffraction, your results are
not that much different from those seen in virus structu
nanoparticles).
Have you looked for such effects in the diffraction patterns which you already
have from the single crystals?
Colin
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of anna anna
Sent: 07 May 2012 17:30
To: ccp4bb
Subject: [ccp4bb] saxs on xtals
Dear all,
I'd like
Dear Anna,
The reprint:-
http://www.sciencedirect.com/science/article/pii/S0020169300822689
has some bearing on your query.
Best wishes,
John
Prof John R Helliwell DSc
On 7 May 2012, at 17:30, anna anna wrote:
> Dear all,
> I'd like some suggestions/opinions about the sense of an experiment
cp4bb
Subject: Re: [ccp4bb] saxs on xtals
Dear Anna,
I once modified CNS to refine two solvent regions of ferritin, one inside and
one outside the shell. Perhaps this can be done in Phenix now. If you want to
locate magnetite particles in this way, you should collect data to as low a
resoluti
: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of anna anna
Sent: Monday, May 07, 2012 12:30 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] saxs on xtals
Dear all,
I'd like some suggestions/opinions about the sense of an experiment proposed by
a collaborator expert in saxs.
In
Sent: Monday, May 07, 2012 5:30 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] saxs on xtals
Dear all,
I'd like some suggestions/opinions about the sense of an experiment proposed by
a collaborator expert in saxs.
In few words, he wants to collect SAXS data on a suspension of protei
It might be that the "bulk solvent correction" is nullifying the interior
of the ferritin structure, and there should be a way to tell the refinement
software not to treat the interior as solvent. Perhaps then you might find
your Fe? Also, I would think there should be some powder-like diffraction
2012/5/7 David Schuller :
> That sounds like powder diffraction.
That was also my impression. There are at least two groups doing
interesting things on this subject - Andy Fitch/Irene Margiolaki at
ESRF and Robert von Dreele at APS. I've contacted the first group
after meeting Andy Fitch in a XRD
That sounds like powder diffraction.
On 05/07/12 12:30, anna anna wrote:
Dear all,
I'd like some suggestions/opinions about the sense of an experiment
proposed by a collaborator expert in saxs.
In few words, he wants to collect SAXS data on a suspension of protein
xtals to investigate "low res
Dear all,
I'd like some suggestions/opinions about the sense of an experiment
proposed by a collaborator expert in saxs.
In few words, he wants to collect SAXS data on a suspension of protein
xtals to investigate "low resolution periodicity" of the xtal (more details
below).
The experiment requires
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