Dear All:
I am sorry that I did not know the policy.
And thanks a lot for the kind reminder.
Jerry
CC: CCP4BB@JISCMAIL.AC.UK
From: [EMAIL PROTECTED]
Subject: Re: [ccp4bb] FW: [ccp4bb] salt sensitive complex
Date: Thu, 31 Jan 2008 09:37:01 +0100
To: [EMAIL PROTECTED]
Dear all
Dear all -Sorry to intervene on a 'book keeping' issue, but indeed over the last few months an increasing number of people (Jerry is not the first, so Jerry please do not take it personally) attach pictures etc. I think in a bb standard practice dictates to only use text - if illustrations are need
Hi Jerry,
I believe that if you make your cuvette and sample buffers exactly the same,
you would really cut down on noise, and get an accurate reading. Did you do
that, or is it possible?
Jacob
***
Jacob Pearson Keller
Northwestern University
Medical Sci
Dear All:
Firstly I would like to thank many folks here for giving me great ideas
several days ago.
The following are some updates for this question.
I did ITC experiments again using 25mMTris(pH8), 60mM NaCl(low salt
condition).
But things still turn out to be a littl
Hi Jerry,
to summarise your problem, using (close to) physiological buffer, SPR
and ITC give you different results, you get different results in
different salt strengths and to add to your misery, the proteins
precipitate at low salt concentrations when mixed to together.
Ok.
Given the above, yo
Dear Jerry,
Are there some better ways that I can validate the binding affinity?
I think it is possible to calculate the interacting energies by
APBS... once you have the complex structure this is! Would be
interesting to compare the values of the constants you get from ITC
and SPR with
Dear All:
Recently I am pursuing the crystallziation of a complex formd by two
individual proteins and I met several interesting problems though they are
kind of off-topic.
Any suggestions for these problems will be highly appreciated.
BIAcore showed about submicro
