Thanks a lot for all your suggestions.
But how much DTT or BME shall i add. Also i fear that these will interfere
in the formulation and how shall i get rid of them then. As for GPC my
protein is 19KDa and diemr will be approx 38 KDa and it is difficult to
separate them without losing the protein
Why don't you add some reducing agent like DTT or BME to make everything as
monomer?
Anthony
On Tue, 17 Feb 2009 10:47:28 +, Meg wrote
> I have purified my protein granulocyte colony stimulating factor using
> chromatography steps. my protein is relatively pure when analysed by
> reducing
I have purified my protein granulocyte colony stimulating factor using
chromatography steps. my protein is relatively pure when analysed by
reducing and non-reducing SDS PAGE method. however non-reducing page
shows DIMER presence. i have about 250 ml pure protein sample and do not
want to perfo
