Why don't you add some reducing agent like DTT or BME to make everything as monomer?
Anthony On Tue, 17 Feb 2009 10:47:28 +0000, Meg wrote > I have purified my protein granulocyte colony stimulating factor using > chromatography steps. my protein is relatively pure when analysed by > reducing and non-reducing SDS PAGE method. however non-reducing page > shows DIMER presence. i have about 250 ml pure protein sample and do not > want to perform gel permeation chromatography as it will be time consuming. > is there a way to get rid of dimers by some other method. can dialysis help > me. kindly guide me as i have to formulate the product and fill it quickly. > > Thanks in anticipation. > > meg ------------------------------------------------- Anthony Addlagatta, Ph.D. Ramanujan Fellow and Senior Scientist Center for Chemical Biology Indian Institute of Chemical Technology [IICT] Tarnaka, Hyderabad- 500007, INDIA Tel:+91-40-27191583 Url: http://www.iictindia.org/zacb/Dr.%20Anthony.aspx
