Dear Eleanor,
The cell dimensions of the dataset with tNCS are 63.995 75.459 128.860
90.00 90.00 90.00 in P 2 21 21 space group.
But for the other dataset without tNCS of the same protein, the cell
dimensions are 64.203 65.319 76.443 90.00 90.00 90.00 in C 2 2 21.
So the cell volume
Are your unit cell and SG correct? I think you maybe should reindex to get a
cell volume 25% of this one, and maybe SG P21
That patterson peak is enormous..
Eleanor
On 1 Aug 2012, at 08:45, Qixu Cai wrote:
> Dear Randy,
>
> Thanks very much for your detailed explanation and helpful advice. I hav
Dear Randy,
Thanks very much for your detailed explanation and helpful advice. I have
run the phaser job just as you have said. This is the result.
The resolution of this dataset is 2.45A.
=
I added the three commands to phaser
Hi,
The second Patterson peak is twice the first (considering lattice translations,
where 1 is equivalent to 0 modulo 1), and then if you triple the first vector
you'll get minus the first vector (again considering lattice translations, i.e.
3/4 is equal to 1 - 1/4 which is equivalent to
-1/4)
It's a P212121 dataset. I have used phaser to find four solution in ASU.
This is the phaser log file:
PEUDO-TRANSLATIONAL NCS VECTOR
--
Space Group : P 21 21 21
Pat
My understanding is that ML functions in the presence of pseudo-translational
symmetry are suboptimal (see a very short discussion in Acta Cryst. (2011).
D67, 355–367. Acta Cryst. (2008). D64, 99–107 is also a good reading).
Having said that if your crystal/dataset exhibits twinning on top of
ps
Dear all,
Can I use the "twin refinement" to refine the pesudo-translational symmetry
dataset?
Thanks a lot for your help.
Best wishes,
Qixu Cai
