09
Please respond to "Satheesh Kumar Palani Nathan" <[EMAIL PROTECTED]>
To
CCP4BB@JISCMAIL.AC.UK
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[ccp4bb] purification of phosphorylated proteins-off topic
Dear CCP4BB,
This is an off topic question for CCP4BB, I am posting on behalf of my
friend:
Hi,
I
I am trying to find a method to purify/separate phosphorylated protein
from unpshosphorylated proteins. I have two approach in my mind
* ion-exchange
* Fe columns, I tried this too but limited success.
Gadolinuim-charged IDA with a shallow gradient of imidazole is supposed to
separate ph
Hi there Satheesh,
In:
Activation Mechanism of the MAP Kinase ERK2 by Dual Phosphorylation .
Cell , Volume 90 , Issue 5 , Pages 859 - 869
B . Canagarajah , A . Khokhlatchev , M . Cobb , E . Goldsmith
(PDB # 2ERK)
the structure of phosphorylated ERK II was solved by co-expressing a
suitable, cons
Dear CCP4BB,
This is an off topic question for CCP4BB, I am posting on behalf of my friend:
Hi,
I am trying to find a method to purify/separate phosphorylated protein from
unpshosphorylated proteins. I have two approach in my mind
ion-exchange
Fe columns, I tried this too but limited
