Re: [ccp4bb] pukka puckers within coot

2015-04-27 Thread Robert Immormino
The Richardson's have another tool for RNA backbone rebuilding which *may* help called RNABC - RNA Backbone Correction. http://kinemage.biochem.duke.edu/software/rnabc.php On Thu, Apr 16, 2015 at 8:22 AM, Almudena Ponce Salvatierra < maps.fa...@gmail.com> wrote: > Dear all, > > Despite molprobit

Re: [ccp4bb] pukka puckers within coot

2015-04-16 Thread Francis Reyes
Sounds like your refinement is failing probably because of the low data:parameter ratio at this resolution and/or the composition of your asymmetric unit, poor initial phase information (probably a molecular replacement solution which is close, but still far from the truth either in sequence co

[ccp4bb] pukka puckers within coot

2015-04-16 Thread Almudena Ponce Salvatierra
Dear all, Despite molprobity analysis tells me my structure contains no puckers that are wrong, the pukka puckers tool within coot lists a number of them with for example inconsistent distance bewteen the phosphates. I would like to ask if any of you have dealt with this before, and how does one