Dear all, Despite molprobity analysis tells me my structure contains no puckers that are wrong, the pukka puckers tool within coot lists a number of them with for example inconsistent distance bewteen the phosphates.
I would like to ask if any of you have dealt with this before, and how does one "make consistent" the distance between phosphates with the sugar puckering? Only by direct space refinement? Or is there a way to change something else... My structure is a 3 A resolution so I can definitely see the backbone of the nucleic acid chain but for sure I can not tell whether the pucker was assigned properly or not from the electron density. Any ideas? Thanks a lot in advance. Best wishes, Almudena. -- Almudena Ponce-Salvatierra Macromolecular crystallography and Nucleic acid chemistry Max Planck Institute for Biophysical Chemistry Am Fassberg 11 37077 Göttingen Germany
