gt;
> Best,
>
> shijun
>
>
>
> -原始邮件-
> 发件人:"Nikolay Dobrev"
> 发送时间:2020-07-19 20:29:49 (星期日)
> 收件人: CCP4BB@JISCMAIL.AC.UK
> 抄送:
> 主题: Re: [ccp4bb] protein oligomer
>
> It really depends from the nature of the protein and if is
> ol
Hi,
low expression levels could indicate (just a few thoughts):
- the protein is not folded properly and already degraded during
production which in turn may indicate problems with the construct
(insert + plasmid) itself or problems with the speed of expression (i.e.
no time to fold). Lower t
thias
From: CCP4 bulletin board on behalf of 张士军
<21620150150...@stu.xmu.edu.cn>
Sent: Monday, July 20, 2020 8:38:46 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] protein oligomer
Dear:
The protein was purified in 4 degree, and the expression level is
arginine when purification, or
do mutantions. anyone has website for prediction the mutation sites of protein?
Thanks!
Best,
shijun
-原始邮件-
发件人:"Nikolay Dobrev"
发送时间:2020-07-19 20:29:49 (星期日)
收件人: CCP4BB@JISCMAIL.AC.UK
抄送:
主题: Re: [ccp4bb] protein oligomer
It really depend
It really depends from the nature of the protein and if is
oligomerizing/agregating/forming polymers if any of this is reversible.
On the other side if you are working with one of the fibril forming
protein it will require optimizaiton on its on as they will form
naturally polymers.
Do you obs
Keep the protein concentration low during purification steps along with using
other anti-aggregation agent/s. Make sure that the pH at which you are
purifying is not close to the pI of the protein. Until completely purified, all
purification steps should be performed in a cold room if it is a so
I am not sure what you mean by polymer formation. Presuming that you have
optimized your protein concentration, pH and salt concentration, you could
try arginine as an anti-aggregation agent in your purification (I presume
you do FPLC). Have a look at chaotropic agents used in protein
purification,
Dear all:
Any ideas to decrease protein polymer formation? my protein was easy to form
oligomers and precipitation when do purification,I have tried add glycerol and
DTT,both didn't work. Does anyone has experience to avoid it happening. Thanks!
Best Regards
###
