tein solution used for
>> crystallization.
>>
>> Herman
>>
>> ----------
>> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
>> *John Domsic
>> *Sent:* Wednesday, January 16, 2013 2:22 PM
>> *To:* CCP4
*From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *John
> Domsic
> *Sent:* Wednesday, January 16, 2013 2:22 PM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] protein degradation in crystal
>
> Hi Lisa,
>
> Speed is definitely a big factor here. With
used for crystallization.
Herman
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On
Behalf Of John Domsic
Sent: Wednesday, January 16, 2013 2:22 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] protein degradation in
Were the crystals which run different on gels from the same drop, or
separate drops?
Yes, it is possible that the protein is being cleaved in the crystal
(self-cleavage?); but it may also be that it is being cleaved in the
mother liquor, and that crystallization is enriching one form or
anoth
Were the crystals which run different on gels from the same drop, or
separate drops?
Yes, it is possible that the protein is being cleaved in the crystal
(self-cleavage?); but it may also be that it is being cleaved in the
mother liquor, and that crystallization is enriching one form or
anoth
Hi Lisa,
Speed is definitely a big factor here. With a protein I work with I can
get large crystals in myriad conditions that only diffract to about 4-5
Ang. What I ended up doing was taking these crystals and seeding entire
screens. I found that not only would crystals appear sooner but it
rev
Dear Lisa
It is not uncommon to see breakdown products when you run crystals on
gel. Espesially if they are older crystals, sometimes you even see
higher molecular bands, these are probably due to intra molecular cross
links formed over time.
If you are worried about stability, try to increase
Hi All,
I have an 36KD protein which can be crystallize in two days. Most of the
crystals are very big. But all cystals have poor resolution,lower than 3.8
A. I picked some crystals, washed them in the mother solution and then run
SDS-PAGE. It is surprised to find that different cystals have differ
