Re: [ccp4bb] protein crystal decay problem

2008-08-04 Thread Juergen Bosch
I'd rather go for a complete low resolution dataset. Is this a native crystal ? HA derivative ? Even with a 4Å data set you can do something it's painful but not impossible e.g. molecular replacement if possible. Then at least you would know your spacegroup & perhaps gain some insights from

Re: [ccp4bb] protein crystal decay problem

2008-08-04 Thread junfeng liu
How about 90? If it is ok , maybe you need more than 3 or more xtals to collect the whole dataset and merge them together. Otherwise your xtals should be the anisotropic .You'd better to collect images in the better region or screen more xtals to get better ones. Good luck! leo lei feng wrote:

[ccp4bb] protein crystal decay problem

2008-08-04 Thread lei feng
hello everyone, I am seeking help on a crystal decay problem I got crystals of a protein , but it decayed very fast in syncrotron beam. From the initial index, it is P222 space group, I need about 180 images, its initial diffraction went to about 3 A, and after 70-80 images, it droped to abo