hello everyone, I am seeking help on a crystal decay problem
I got crystals of a protein , but it decayed very fast in syncrotron beam. From
the initial index, it is P222 space group, I need about 180 images, its initial
diffraction went to about 3 A, and after 70-80 images, it droped to about 6. I
already used low exposure time and it is in cryo , what I can do ? any
suggestion is appreciated.
BTW. the condition of crystallization is 0.1M NaAC, pH 5.5, 1 M LiCl,
15% PEG 6000,
I used 5 seconds / image , which can go to 3 A, if I use 2 seconds, the
initial resolution is only 3.5 A ( beam energy 12kev)
Thanks
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