Hi Steve,
You should definitively follow Jürgen's suggestion and try to solve the
structure. If you could get an idea about the crystal packing it would
help you to optimize your DNA construct. This is often critical in
protein-nucleic acid crystallization. You could try to change the length
of you
W.M. B. wrote:
Dear All:
My protein/DNA complex crystal diffracts to 3.2 A ( in-house source).
It crystalized in 20% PEG 8K, NaCaCo 6.5. I tried to add ATP, CaCl2
and spermidine. The diffaction doesn't improve. Would you please give
me some advice?
Thanks a lot,
Steve
How does
Dear All:
My protein/DNA complex crystal diffracts to 3.2 A ( in-house source). It
crystalized in 20% PEG 8K, NaCaCo 6.5. I tried to add ATP, CaCl2 and
spermidine. The diffaction doesn't improve. Would you please give me some
advice?
Thanks a lot,
Steve
