[ccp4bb] Peptide crystallization

Dear All, I have a small synthesized peptide(3kD). I want to crystallize the peptide. Should I follow the same way as protein crystallization? Can anybody suggest me? Thanks in advance, Dipankar Manna This e-mail and any files transmitted with it are for the s

Re: [ccp4bb] peptide crystallization question

Dear Ritcha, we have crystallised various peptides, especially circular ones, both from regular screens used in MX and from specially prepared screens (i.e. different organic solvents). We tend to use slow evaporation from under mineral oil in Terazaki plates (i.e. microbatch). We have also fa

Re: [ccp4bb] peptide crystallization question

On Tue, 2011-09-06 at 11:51 -0500, Chaudhary, Ritcha wrote: > Do people use regular screens used in macromolecular crystallization > such as Hamptons, wizards etc? Probably not - I believe short peptides are crystallized mostly via various evaporation techniques (but I am very curious to hear what

[ccp4bb] peptide crystallization question

Dear all I am interested in crystallizing a 5 residue peptide. I have no prior experience in this field although I have crystallized larger proteins (5--60 kDa). Do people use regular screens used in macromolecular crystallization such as Hamptons, wizards etc? Any suggestions are greatly app

Re: [ccp4bb] Peptide Crystallization

[mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Buz Barstow Sent: Wednesday, May 25, 2011 8:04 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Peptide Crystallization Dear all, I am considering trying to crystallize a small peptide (around 15 amino acids). The peptide is soluble in neutral water or buffer (pH

Re: [ccp4bb] Peptide Crystallization

I would discourage using pre-made screens on a project outside the norm. The main problems are: Zinc: At mM concentrations will easily crystallize and give false positives in many screens. It will also act as a precipitant for the peptide. The best approach would be to separate the zinc adduct

Re: [ccp4bb] Peptide Crystallization

NMR ... synthesize with a few labeled aa according to taste of local NMR guru. If they cant do a15-aa peptide in a day, let the bb know, it will be entertaining ;-) A. On May 25, 2011, at 14:04, Buz Barstow wrote: Dear all, I am considering trying to crystallize a small peptide (around 15

[ccp4bb] Peptide Crystallization

Dear all, I am considering trying to crystallize a small peptide (around 15 amino acids). The peptide is soluble in neutral water or buffer (pH 7.0) until at least 10 mM (13 mg/mL), and adopts a turn conformation when bound to Zn. What are your thoughts on attempting this? If you think that i

Re: [ccp4bb] peptide crystallization

There was a brief discussion of this on the board last year. See: http://www.dl.ac.uk/list-archive-public/ccp4bb/msg18757.html Good luck kmj shivesh kumar wrote: Dear all, Can u suggest me about the peptide crystallization,some references.Should I try it with PEG or MPD.It is 10-15 residues

[ccp4bb] peptide crystallization

Dear all, Can u suggest me about the peptide crystallization,some references.Should I try it with PEG or MPD.It is 10-15 residues long.Thanx in advance. S...