NMR ... synthesize with a few labeled aa according to taste of local
NMR guru.
If they cant do a15-aa peptide in a day, let the bb know, it will be
entertaining ;-)
A.
On May 25, 2011, at 14:04, Buz Barstow wrote:
Dear all,
I am considering trying to crystallize a small peptide (around 15
amino acids). The peptide is soluble in neutral water or buffer (pH
7.0) until at least 10 mM (13 mg/mL), and adopts a turn conformation
when bound to Zn.
What are your thoughts on attempting this?
If you think that it is worthwhile, what crystallization conditions
would you try? I am thinking of a sparse matrix screen using the
Hampton Crystal Screen 1 and 2 kits, using hanging drop
crystallization in Hampton Vdx trays.
Thanks! and all the best,
---Buz
P please don't print this e-mail unless you really need to
Anastassis (Tassos) Perrakis, Principal Investigator / Staff Member
Department of Biochemistry (B8)
Netherlands Cancer Institute,
Dept. B8, 1066 CX Amsterdam, The Netherlands
Tel: +31 20 512 1951 Fax: +31 20 512 1954 Mobile / SMS: +31 6 28 597791
