g surprise, sometimes.
Yu Xiaodi
Date: Wed, 8 Feb 2012 11:56:30 +0530
From: somon_...@yahoo.co.in
Subject: [ccp4bb] pH optimisation for crystallisation
To: CCP4BB@JISCMAIL.AC.UK
Dear all,
I have a 17 KDa protein that gives crystals in a condition that has
sometimes.
Yu Xiaodi
Date: Wed, 8 Feb 2012 11:56:30 +0530
From: somon_...@yahoo.co.in
Subject: [ccp4bb] pH optimisation for crystallisation
To: CCP4BB@JISCMAIL.AC.UK
Dear all, I have a 17 KDa protein that gives crystals in a condition
that has 0.1M bis-tris pH 6.5. The crystals are t
Dear Sreetama,
First of all, there are no hard-and-fast "rules" for successful
crystallisation, try changing as many different variables as possible
and go with what works.
Having said that, yes, next I would go for a grid optimisation varying
the pH in 0.2 or 0.5 units over as wide a range
Dear all,
I have a 17 KDa protein that gives crystals in a condition that has
0.1M bis-tris pH 6.5. The crystals are thin needle clusters and do not
diffract. I have tried additives, but they haven't improved the crystals. I
intend to vary the pH of the condition.
My questi
