this is also my experience. However, above pH 8, the buffering effect of the
Jeffamines' amino groups starts to be significant and (rough) pre-adjusting of
the pH might be necessary.
Clemens
Quoting Yogesh Gupta PhD <[EMAIL PROTECTED]>:
Hi Amit,
Changing the pH of Jeffamine is not so easy . I
Hi Amit,
Changing the pH of Jeffamine is not so easy . I would keep the pH of
Jeffamine constant and rather change the pH of buffer. Anyway if Jeffamine
concentration is too low as compare to the buffer you are using, it would
not change the final pH much. This is what my experience with Jeffamine
Dear CCP4ers,
Apologies for a non-CCP4 query. I intend to set up an optimization grid
around one of the crystallization conditions (containing Jeffamine ED-2001pH
7.0 and HEPES pH 7.0) by varying the buffer pH from 5.5 to 7.5. In such a
case, is it recommmended that the pH of Jeffamine ED-2001(pH
