Dear CCP4ers, Apologies for a non-CCP4 query. I intend to set up an optimization grid around one of the crystallization conditions (containing Jeffamine ED-2001pH 7.0 and HEPES pH 7.0) by varying the buffer pH from 5.5 to 7.5. In such a case, is it recommmended that the pH of Jeffamine ED-2001(pH 7.0) also be changed to match the different buffer pHs(5.5-7.5)?
Cheers, Amit
