This can be very hard to do because quite a few proteases are promiscuous
and will cut substrates solely based on masking of the polypeptide within
the structure of the protein. Typically these proteases will not stop
cutting at a single nick - they often proceed until they can't 'dig into' a
burie
Hi Tom,
You can try the MEROPS database. There is a search feature called "What
peptidases can cleave this bond".
http://merops.sanger.ac.uk/cgi-bin/specsearch.pl
Cheers,
Jack
On Thu, Mar 31, 2011 at 6:59 AM, Brett, Thomas wrote:
> Hi all:
> I was wondering if anyone had any tips on identify
Hi all:
I was wondering if anyone had any tips on identifying proteases. I have a
protein for which I know the proteolytic cleavage site. What are the best ways
to identify the protease that does the cutting either:
1) bioinformatically (i.e., a good database to search using the cleavage site
or
