Hi all: I was wondering if anyone had any tips on identifying proteases. I have a protein for which I know the proteolytic cleavage site. What are the best ways to identify the protease that does the cutting either: 1) bioinformatically (i.e., a good database to search using the cleavage site or a consensus) 2) experimentally (some engineered substrate to trap/identify the substrate or any other method?) Thanks in advance -Tom
Tom J. Brett, PhD Assistant Professor of Medicine Division of Pulmonary and Critical Care Washington University School of Medicine Campus Box 8052, 660 S. Euclid Saint Louis, MO 63110 http://brettlab.dom.wustl.edu/
