ge
> maybe if your crystallization experiments give you some more hints about the
> solubility of the protein.
>
> Good luck,
> Jan
>
>
> --- Katja Schleider ** schrieb am *Do,
> 19.11.2009:
> *
>
> *
> Von: Katja Schleider
> Betreff: [ccp4bb] off-topic: crystal opt
might change maybe if your
crystallization experiments give you some more hints about the solubility of
the protein.
Good luck,
Jan
--- Katja Schleider schrieb am Do, 19.11.2009:
Von: Katja Schleider
Betreff: [ccp4bb] off-topic: crystal optimization without buffer
An: CCP4BB@JISCMAIL.AC.UK
Hi Katja,
Sodium sulphate forms a solution of neutral pH in water.
200 mM of Na2SO4 will buffer the crystallization solution at pH 7, in
addition to acting as a salt. You could try varying the concentration of
PEG and Sod.sulphate now to see if the crystals get better. Sometimes using
potassiu
Forgot to hit the "Reply all" button (sorry :-( )
--- Begin Message ---
Katja Schleider wrote:
Hi everybody,
sorry for my off-topic question. I got small initial crystals in 200mM
NaSulfat and 20% PEG 3350.
There is no buffer in this condition. How can I optimize these
crystals? Just vary the
Hi everybody,
sorry for my off-topic question. I got small initial crystals in 200mM NaSulfat
and 20% PEG 3350.
There is no buffer in this condition. How can I optimize these crystals? Just
vary the PEG concentration? Or should I add a buffer; or vary the pH of the
buffer the proteinsolution w
