Hi Katja,
it makes perfect sense to add a buffer to your assay. Of course for the
beginning something which buffers well around pH 7 like HEPES, BTP, etc. would
be appropriate. If your purification protocol is optimized I would not change
the conditions of the purification buffer. But this might change maybe if your
crystallization experiments give you some more hints about the solubility of
the protein.
Good luck,
Jan
--- Katja Schleider <katjaschlei...@yahoo.de> schrieb am Do, 19.11.2009:
Von: Katja Schleider <katjaschlei...@yahoo.de>
Betreff: [ccp4bb] off-topic: crystal optimization without buffer
An: CCP4BB@JISCMAIL.AC.UK
Datum: Donnerstag, 19. November 2009, 10:33
Hi everybody,
sorry for my off-topic question. I got small initial crystals in 200mM NaSulfat
and 20% PEG 3350.
There is no buffer in this condition. How can I optimize these crystals? Just
vary the PEG concentration? Or should I add a buffer; or vary the pH of the
buffer the proteinsolution was in?
Thank you and best regards,
Katja
