te:
I'll swap you for the deamination, as that pertains to lysines...it's
been a long week :)
Steve
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Nadir T. Mrabet
Sent: Friday, February 18, 2011 2:04 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: R
look like?
>>> Cheers,
>>> Steve
>>>
>>> ------------------------
>>> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On
>>> Behalf Of *Ulli Hain
>>> *Sent:* Friday, February 18, 20
--
>> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On
>> Behalf Of *Ulli Hain
>> *Sent:* Friday, February 18, 2011 12:14 PM
>> *To:* CCP4BB@JISCMAIL.AC.UK
>> *Subject:* [ccp4bb] off-topic: 2 peaks on Cation
&
e: [ccp4bb] off-topic: 2 peaks on Cation
Typo! I actually meant deamidation.
Nadir
Pr. Nadir T. Mrabet
Structural& Molecular Biochemistry
Nutrigenex - INSERM U-954
Nancy University, School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 Vandoeuvre-les-Nancy Cedex
France
Phone: +33 (0
Typo! I actually meant deamidation.
Nadir
Pr. Nadir T. Mrabet
Structural& Molecular Biochemistry
Nutrigenex - INSERM U-954
Nancy University, School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 Vandoeuvre-les-Nancy Cedex
France
Phone: +33 (0)3.83.68.32.73
Fax: +33 (0)3.83.68.32.79
E
P4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] off-topic: 2 peaks on Cation
Hi, I was wondering if anyone had possible explanations for a
recombinantly expressed soluble protein that runs as 2 equal, slightly
overlapping peaks on a cation exhanger but as one peak on a size
exclusion column and same electrophor
@JISCMAIL.AC.UK
Subject: [ccp4bb] off-topic: 2 peaks on Cation
Hi, I was wondering if anyone had possible explanations for a
recombinantly expressed soluble protein that runs as 2 equal, slightly
overlapping peaks on a cation exhanger but as one peak on a size
exclusion column and same electrophoretic
Hi,
you did not mention anything about your protein, but if it shows a metal
dependency, than different amounts of the metal ion might changen the overall
charge and influence the interactions with the ion exchanger. There might be
also a modification of an aminoacid f.e. decarboxylation of an a
Hi, I was wondering if anyone had possible explanations for a recombinantly
expressed soluble protein that runs as 2 equal, slightly overlapping peaks on
a cation exhanger but as one peak on a size exclusion column and same
electrophoretic mobility on SDS-PAGE.
-Ulli
Adelaide Ulricke Hain
Ph
