I'll swap you for the deamination, as that pertains to lysines...it's been a long week :)
Steve -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Nadir T. Mrabet Sent: Friday, February 18, 2011 2:04 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] off-topic: 2 peaks on Cation Typo! I actually meant deamidation. Nadir Pr. Nadir T. Mrabet Structural& Molecular Biochemistry Nutrigenex - INSERM U-954 Nancy University, School of Medicine 9, Avenue de la Foret de Haye, BP 184 54505 Vandoeuvre-les-Nancy Cedex France Phone: +33 (0)3.83.68.32.73 Fax: +33 (0)3.83.68.32.79 E-mail: Nadir.Mrabet<at> medecine.uhp-nancy.fr On 18/02/2011 20:36, Christian Roth wrote: > Hi, > you did not mention anything about your protein, but if it shows a metal > dependency, than different amounts of the metal ion might changen the overall > charge and influence the interactions with the ion exchanger. There might be > also a modification of an aminoacid f.e. decarboxylation of an aspartate or > glutamate. > > Christian > > Am Freitag 18 Februar 2011 18:13:36 schrieb Ulli Hain: >> Hi, I was wondering if anyone had possible explanations for a >> recombinantly expressed soluble protein that runs as 2 equal, slightly >> overlapping peaks on a cation exhanger but as one peak on a size exclusion >> column and same electrophoretic mobility on SDS-PAGE. -Ulli >> >> Adelaide Ulricke Hain >> PhD Candidate >> Johns Hopkins Bloomberg School of Public Health >> Department of Biochemistry and Molecular Biology >> 615 North Wolfe Street >> Baltimore, MD 21205 >> Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
