Hi Anita,
I have recently performed it following this protocol I found in the Internet:
http://wolfson.huji.ac.il/purification/Protocols/PAGE_Acidic.html
I hope this helps! Good luck,
Federica
--
Federica Basilico
Ph.D. student
Department of Experimental Oncology
European Institute
I have actually done this by running a normal PAGE gel without
stacking gel and switching the electrodes, which seemed to work
swimmingly.
JPK
On Thu, Jan 19, 2012 at 9:25 AM, Katherine Sippel
wrote:
> Hi Rashmi,
>
> In my experience native (even blue native) on proteins around that pI is
> sket
Hi Rashmi,
In my experience native (even blue native) on proteins around that pI is
sketchy at best. The electrophoretic mobility once it gets past the
stacking gel goes to crap meaning long electrophoresis times and it needs
to be done on a chillable system or in a cold room. If this is a multime
Le 19/01/12 14:03, anita p a écrit :
> Hi All,
> Has anyone run a native gel for proteins at pI>8 .
> I want to pour my own native gel. Do I run a discontinuous page or a
> continuous one?? Please help with regards to the buffer system to be
> used, and the dye to be used.
> With regards
> Rashmi
Hi All,
Has anyone run a native gel for proteins at pI>8 .
I want to pour my own native gel. Do I run a discontinuous page or a
continuous one?? Please help with regards to the buffer system to be used,
and the dye to be used.
With regards
Rashmi
