Le 19/01/12 14:03, anita p a écrit : > Hi All, > Has anyone run a native gel for proteins at pI>8 . > I want to pour my own native gel. Do I run a discontinuous page or a > continuous one?? Please help with regards to the buffer system to be > used, and the dye to be used. > With regards > Rashmi
Hi Rashmi, Try the blue-native method: Peltier, J. B., Ytterberg, J., Liberles, D. A., Roepstorff, P., and van Wijk, K. J. (2001) Identification of a 350-kDa ClpP protease complex with 10 different Clp isoforms in chloroplasts of Arabidopsis thaliana. J. Biol. Chem., 276: 16318–16327. Wittig, I., Braun, H.-P., and Schagger, H. (2006) Blue native PAGE. Nat Protoc, 1: 418–428. Best regards, -- Miguel Architecture et Fonction des Macromolécules Biologiques (UMR7257) CNRS, Aix-Marseille Université Case 932, 163 Avenue de Luminy, 13288 Marseille cedex 9, France Tel: +33(0) 491 82 55 93 Fax: +33(0) 491 26 67 20 mailto:miguel.ortiz-lombar...@afmb.univ-mrs.fr http://www.afmb.univ-mrs.fr/Miguel-Ortiz-Lombardia
