Re: [ccp4bb] monomeric coiled coil--updated

2010-07-05 Thread Anastassis Perrakis
iation characteristics. 5. There are papers on capillary electrophoresis methods where they study exactly these effects (time of equilibrium vs time of separation). A. Maia - Original Message - From: "aidong" To: Sent: Sunday, July 04, 2010 3:01 AM Subject: Re: [cc

Re: [ccp4bb] monomeric coiled coil--updated

2010-07-04 Thread chern
Message - From: "aidong" To: Sent: Sunday, July 04, 2010 3:01 AM Subject: Re: [ccp4bb] monomeric coiled coil--updated > In light of several wonderful responses,I would like to provide an > update for this question: > > 1. I would agree that SEC might not be able

Re: [ccp4bb] monomeric coiled coil--updated

2010-07-04 Thread aidong
In light of several wonderful responses,I would like to provide an update for this question: 1. I would agree that SEC might not be able to identify monomer vs multimer forms for this likely rod-shaped protein. 2. It is extremely low kd for dimer. AUC and SAXS experiments have measured it