Re: [ccp4bb] molecular replacement in phaser

2009-11-10 Thread Phoebe Rice
ns, may confuse the software because the DNA is denser. Of course, every case has its own idiosyncracies. Phoebe Original message >Date: Tue, 10 Nov 2009 14:19:43 -0500 >From: Christian Biertuempfel >Subject: Re: [ccp4bb] molecular replacement in phaser >To: CCP4B

Re: [ccp4bb] molecular replacement in phaser

2009-11-10 Thread Christian Biertuempfel
Hi Lisa, There are many things you can try and the phaser manual gives a lot of useful information what to do in difficult cases. From my experience, it is quite difficult to find solutions for MR with nucleic acids. I recommend to search only for protein. As a side effect you can use this approach

[ccp4bb] molecular replacement in phaser

2009-11-10 Thread Lisa Wang
Hi all, I got one data about 3.0 A, belong to C2 space group. There are two protein molecules and one 18-nt dsRNA per ASU. The structure of last 100aa (C-terminal) has been reported, and 400 aa at N-terminalhe has homology structure with sequence identiy 30%. I try to solve it by MR with phaser. I