Hi all, I got one data about 3.0 A, belong to C2 space group. There are two protein molecules and one 18-nt dsRNA per ASU. The structure of last 100aa (C-terminal) has been reported, and 400 aa at N-terminalhe has homology structure with sequence identiy 30%. I try to solve it by MR with phaser. I did rotation and tranlation with 18-nt ideal dsRNA first, then search with C-terminal model. I got about 20 sol after seaching dsRNA with TFZ 8-10.0. I use all of them to serch the first c-terminal, and second C-terminal. Ding this process, LLG keep on increasing, and TFZ is higher than 10. But all have serious clash. It looks like all the sol are wrong.
Please give me a hand.
