Dear Kumar
Could you please provide some information on your refinement protocol/progress
and quality of your partial model thus far. Also, what is the quality and
completeness of your data, in particular in the lowest resolution to 10 angs
range. In this way one might be able to provide more
On Thu, 2011-12-08 at 12:17 +0530, atul kumar wrote:
> I can't build anything in this region,this could be because of
> disordered structure or because of low resolution.
Both, and also the presence of disordered fragment may be the reason the
resolution is low, thus the already mentioned suggesti
Hmm- I wonder if B-factor unsharpening (applying a large POSITIVE B-factor to
the data)
would have the same effect?
Francis E Reyes wrote:
On the other hand, shooting a lower resolution crystal may get you the
conformation of the disordered domain.
Surprising at first thought, but was true in
On the other hand, shooting a lower resolution crystal may get you the
conformation of the disordered domain.
Surprising at first thought, but was true in p97/VCP from the Brunger Lab.
F
On Dec 7, 2011, at 11:47 PM, atul kumar wrote:
> Dear all
>
> I have crytals which diffract up
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Dear Atul,
the "resolution" of your data set would affect the whole unit cell and
not just the region of 30-40 residues. The more likely reason is a
disordered N-terminus. There is nothing to do about this in silico.
It may be worth recloning your pr
Dear all
I have crytals which diffract up to 3.2 A at synchrotron, I am solving this
by molecular replacement. I have built 70% of the model successfully,but
the problem is it have a very poor density for some 30-40 residues at N
terminal. I can't build anything in this region,this could be becaus
