lundi 2 mars 2015 10:24
À : CCP4BB@JISCMAIL.AC.UK
Objet : Re: [ccp4bb] merge weak anomalous signal from multiple datasets
Hi Charles,
Actually I also tried the method of Q. Liu et al and got one structure solved
after merging 3 different datasets. The only thing different in my case is the
Se-SAD phasin
Hi Charles,
Actually I also tried the method of Q. Liu et al and got one structure
solved after merging 3 different datasets. The only thing different in my
case is the Se-SAD phasing instead of Br. But the signal was also weak due
to the disorder of incorporated Se in the protein crystals (I supp
Hi Charles,
I don't know what multiscale does. Probably the right thing. If the
anomalous signal is weaker after scaling of multiple datasets,
non-isomorphism might be at fault. Try Blend to scale your datasets.
Blend is part of ccp4 and gives good graphical diagnostic feedback.
Andreas
Dear CCP4 users,
Recently, I got some datasets with weak anomalous Br signal. I tried to
merge them according to Q. Liu et al Science 336, p1033 (2012). I am using
the script multiscale@SSRL. The merged dataset has WEAKER anomalous
signals.
Liu et al used SCALA for scaling and merging while mult
