Hi Charles,
I don't know what multiscale does. Probably the right thing. If the
anomalous signal is weaker after scaling of multiple datasets,
non-isomorphism might be at fault. Try Blend to scale your datasets.
Blend is part of ccp4 and gives good graphical diagnostic feedback.
Andreas
On 01/03/2015 4:20, CPMAS Chen wrote:
Dear CCP4 users,
Recently, I got some datasets with weak anomalous Br signal. I tried to
merge them according to Q. Liu et al Science 336, p1033 (2012). I am
using the script multiscale@SSRL. The merged dataset has WEAKER
anomalous signals.
Liu et al used SCALA for scaling and merging while multiscale@SSRL using
AIMLESS. Should this cause such a difference? The SCALA@SSRL has a
limitation on the number of frames it can process. So I cannot directly
check if this caused the difference.
Any suggestions?
Thanks!
Charles
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Charles Chen
Research Associate
University of Pittsburgh School of Medicine
Department of Anesthesiology
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